Antibody【Antigen:M-O】｜一般社団法人　日本細胞生物学会

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Antibody【Antigen:M-O】

Antibody【Antigen:M-O】

【M】

【m】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
bovine MPR2011-08-12	86f7	WB	IF	IHC	IP
	DSHB
	86f7
	mouse
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

bovine MPR2011-08-12	22d4	WB	IF	IHC	IP
	DSHB
	22d4
	mouse
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

bovine MPR2011-08-10	86f7	WB	IF	IHC	IP
	DSHB		x100
	86f7	3%PFA (in PBS), methanol
	mouse

	マウス小腸
bovine MPR2011-08-10	22d4	WB	IF	IHC	IP
	DSHB		x100
	22d4	3%PFA (in PBS)
	mouse
		マウス小腸はダメ Not suitable for mouse small intestine.
	Caco2, MDCK
c-Myc2011-08-29	9-E10	WB	IF	IHC	IP
	Santa Cruz (BabCO)	x1000	x1000x500

	mouse
	Mono, IgG1	Lot. G050
	Hela細胞
c-Myc2011-08-29	9-E10	WB	IF	IHC	IP
	Santa Cruz (BabCO)	x1000	x1000x500

	mouse
	Mono, IgG1	Lot. G2803
	Hela細胞
C-Myc2011-10-13		WB	IF	IHC	IP
	Santa Cruz		X300
	sc-40
	mouse	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	Mono, IgG1
	HeLa細胞
c-Myc(9E10)2011-10-17		WB	IF	IHC	IP
	Santa Cruz	X500	X500
	sc-40
	mouse	【3.7% formalin solution+0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	Mono	WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	HeLa細胞
CI-M6PR2011-08-29	2G11	WB	IF	IHC	IP
	ABR		x10-20

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a
	Hela細胞
CI-M6PR2011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam
	ab32815
	rabbit
	IgG
	Hela細胞
CI-M6PR (IGFIIR)2011-08-29		WB	IF	IHC	IP
	BD Transduction	x250

	mouse
	Mono	Rat specific, Immunogen: extracellular domain
	Hela細胞
human M6PR2011-08-10		WB	IF	IHC	IP
	GenWay Biotech		x100
	A21444	3%PFA (in PBS), methanol
	chicken(IgY)
	p
	マウス小腸
human MAP LC32011-08-10	H-50	WB	IF	IHC	IP
	Santa Cruz		x100
	sc28266	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
human MAP LC32011-08-10	F-14	WB	IF	IHC	IP
	Santa Cruz		x100
	sc16756	3%PFA (in PBS), methanol
	goat
	p
	マウス小腸
Mab4142011-10-13	Mab414	WB	IF	IHC	IP
	covance	X2000	X500
	MMS-120R
	mouse	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	Mono, IgG1
	HeLa細胞

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
mAb4142014-08-01		WB	IF	IHC	IP
	COVANCE		x400
	MMS-120P	4%PFA/PBS
	mouse
	IgG1
	mouse oocyte (pronucleus)
mAb4142014-08-01		WB	IF	IHC	IP
	COVANCE		x400
	MMS-120P	ice cold Methanol
	mouse
	IgG1
	mouse oocyte (pronucleus)

【m】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
MACF12011-09-29		WB	IF	IHC	IP
	Abnova		X1,000
	H00023499-A01
	Mouse	IF: -20℃ methanol or acetone:methanol=1:1
	Poly	製品のロット間差が大きい
	HeLa細胞
Mad22014-06-04		WB	IF	IHC	IP
	Covance		x500
		3%PFA (in PBS)
	rabbit
	IgG	Make aliquots and store at -20℃. Storing at 4℃ sometimes inactivates this antibody.
	HeLa	Goshima et al., JCB 2003
maltase2012-04-04	HBB2/143/17 monoclonal	WB	IF	IHC	IP
	Developmental Studies Hybridoma Bank		x5
		3%PFA in PBS
	mouse	0.05% saponin
	IgG1	culture sup
	Caco2	JCB 101, 838-851, 1985
Man II2011-08-29	53FC3	WB	IF	IHC	IP
	BAbCO		x1000x500

	mouse
	Mono, IgG2b	Ratしか使えません。 Only applicable for rat cells and tissues.
	Hela細胞
MAP1A2011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	1F4	3%PFA (in PBS)
	mouse
	m
	マウス脳
MAP22011-08-10	clone: HM-2	WB	IF	IHC	IP
	Sigma		x100
	M4403	3%PFA (in PBS)
	mouse
	m
	マウス脳
MAP22011-10-03 About Product	HM-2, monoclonal	WB	IF	IHC	IP
	Sigma-Aldrich		X5000
	M4403
	mouse	【Formalin in PBS+0.05%Triton】:○, 【Formalin in PBS+Methanol】:○,
		ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
MAP22012-12-12		WB	IF	IHC	IP
	Sigma			x500
	M4403	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
		dendrites
	mouse brain
Mash12012-12-12		WB	IF	IHC	IP
	BD pharmingen			x100
		4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
		proneural progenitors
	mouse brain
Math22012-12-12		WB	IF	IHC	IP
	Chemicon		x500
	AB9240	4%PFA (in PBS)
	rabbit	0.1% Triton
	IgG	pyramidal cell
	primary hippocampal neuron
Math2 (NeuroD6)2012-12-12		WB	IF	IHC	IP
	Santa Cruz			x100
	sc-19251	4%PFA (in PBS)
	goat	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG	pyramidal cell
	mouse brain
MDR1+32011-08-10	clone:C219	WB	IF	IHC	IP
	Zymed		x100
	18-7244	methanol
	mouse
		メタノール固定でＯＫ Good staining by methanol fix.
	マウス肝臓
MEK1/22013-09-12	L38C12	WB	IF	IHC	IP
	Cell Signaling Technology	x500
	#4694
	mouse
	IgG1
	HeLa
Mfn1 (human f.l.)2011-10-07	3C9	WB	IF	IHC	IP
	Abnova	500-1000
	H00055669-M04
	mouse
		Mfn1, 2両方を認識するらしい
	HeLa
Mfn2 (human 661-758aa)2011-10-07	4H8	WB	IF	IHC	IP
	Abnova
	H00009927-M03J
	mouse
		Mfn2 specific
	HeLa
MHCI2011-08-29	W6/32	WB	IF	IHC	IP
	ATCC		原液orx10

	mouse	【PFA+Triton】:△,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono	Hybridoma Sup't、Chase後、Fixする前に必ずAcid washすること。 After chase, wash with acid before fix.
	Hela細胞
MITF2011-09-17 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam
	ab13703
	mouse
	IgG1
	Hela細胞
MITF2011-09-28 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam	x1000
	ab13703
	mouse
	IgG1
	HeLa細胞
MKLP12011-09-27		WB	IF	IHC	IP
	Cytoskeleton		100
	AKIN06
	rabbit
	p	TCA, 4%PFA, 1%PFA, MeOH
	HeLa
MKLP12011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-867
	rabbit
	p	TCA, 4%PFA, 1%PFA, MeOH
	HeLa
MKLP1(N-19)2011-08-29	sc-867	WB	IF	IHC	IP
	Santa Cruz	x150	x200-

	rabbit
	Poly
	Hela細胞
MKLP2(Rab6KIFL, Rabkinesin6)2011-08-29		WB	IF	IHC	IP
	BETHYL Laboratories

	rabbit
	Poly
	Hela細胞
MKP22011-08-14		WB	IF	IHC	IP
	BD transduction	x1000
	610850
	mouse
	m
	マウス神経細胞
MLC22011-10-28		WB	IF	IHC	IP
	Cell Signaling		1/500
	#3672
	rabbit
	m
	マウス非筋細胞
MLX2011-09-17 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam
	ab65147
	rabbit
	IgG
	Hela細胞
MLX2011-09-28 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	x250
	ab65147
	rabbit
	IgG
	HeLa細胞
Moesin2011-08-29	38/87	WB	IF	IHC	IP
	Santa Cruz		x50

	mouse
	Mono, IgG1
	Hela細胞
mouse MAP 2a,b,c Ab-32011-08-10		WB	IF	IHC	IP
	NeoMarkers		x100
	MA250	3%PFA (in PBS)
	mouse
	m
	マウス脳
mouse MAP22011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	2G5	3%PFA (in PBS)
	mouse
	m
	マウス脳
mouse metabtropic GluR1a2011-08-10		WB	IF	IHC	IP
	Frontier		x100
	mGluR1aGoAf12201	3%PFA (in PBS)
	goat
	p	ＯＫ（北大渡辺先生作）パパインによる前処理必要 Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
	マウス脳
mouse metabtropic GluR1a2011-08-10		WB	IF	IHC	IP
	Frontier		x100
	mGluR1aGoAf12201	3%PFA (in PBS)
	goat
	p	ＯＫ（北大渡辺先生作）パパインによる前処理必要 Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
	マウス脳
MRP22011-08-10	M2?-5)=MDR2	WB	IF	IHC	IP
	Alexis
	ALX-801-037
	mouse
	m

MSH2011-08-10		WB	IF	IHC	IP
	群大生体調節研 IMCR, Gunma Univ		x100
	HACAA2201RBP84	3%PFA (in PBS)
	rabbit

	マウス下垂体
Myc2011-08-10		WB	IF	IHC	IP
	Upstate
	6340
	rabbit


Myc2011-08-29		WB	IF	IHC	IP
	MBL	x100	x100

	rabbit
	Poly
	Hela細胞
myc2011-09-17	#9E10	WB	IF	IHC	IP
	clontech	x1000

	mouse
	mono
	Hela細胞
myc2011-09-28	#9E10	WB	IF	IHC	IP
	clontech	x1000

	mouse
	mono
	HeLa細胞
myc2011-10-03	PL14	WB	IF	IHC	IP
	MBL		x1000

	mouse	【Formalin in PBS+0.05%Triton】：○, 【Formalin in Ringer液+0.05%Triton】：○
	Mono,IgG1	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	COS細胞,rat hippocampal neurons
myc2011-10-03		WB	IF	IHC	IP
	MBL	x1000			x1000
	562-5
	rabbit
	Poly
	HEK293T細胞,COS細胞,rat hippocampal neurons
Myc-Tag2011-08-14	9B11	WB	IF	IHC	IP
	Cell Signaling	x1000	x100
	2276
	mouse
	m

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Myc-Tag2015-01-13		WB	IF	IHC	IP
	Cell Signaling Technology		x2000
	2276	3%PFA (in PBS)
	Mouse	0.05%saponin
	IgG2a
	MEF

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
MyHC IIB(nonmuscle)2011-09-27		WB	IF	IHC	IP
	Covance		200-500
	PRB-445P
	rabbit
	p	4%PFA, 1%PFA, MeOH, TCA
	MDCK II, EpH4, MTD-1A, DLD-1, HeLa
Myosin IIA, non muscle2011-09-27		WB	IF	IHC	IP
	Sigma		200-500
	M8064
	rabbit
	p	4%PFA, 1%PFA, MeOH, TCA
	MDCK II, EpH4, MTD-1A, DLD-1, HeLa
Myosin phospatase2011-09-27		WB	IF	IHC	IP
	Covance		100
	PRB-457C
	rabbit
	p	1%PFA>TCA
	HeLa
Myosin-B (MHC-B)2011-10-28		WB	IF	IHC	IP
	COVANCE		1/500
	PRB-445P
	rabbit
	m
	マウス非筋細胞
MyosinII a,b,c2011-08-29		WB	IF	IHC	IP
	Plyclone



	Hela細胞
MyosinVB2011-08-10		WB	IF	IHC	IP
	LifespanBioscience		x100
	LS-B2119	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
MYPT12011-10-28		WB	IF	IHC	IP
	Cell Signaling	1/1000
	#2634P
	rabbit
	m, h
	マウス非筋細胞
MYPT1 pT850 (chick) / pT853 (human)2013-02-01		WB	IF	IHC	IP
	Upstate (Millipore)	X1000
	36-003
	rabbit (poly)
		cell lysate調製時に急速に脱リン酸化されるため、TCA処理またはSDS sample bufferによる調製を推奨 Samples should be directly prepared by TCA or SDS sample buffer because of the rapid dephosphorylation in preparation.

Nonmuscle Myosin II heavy chain2011-09-27		WB	IF	IHC	IP
	Covance		100
	MMS-442P
	mouse
	m	蛍光染色不可 Not suitable for IF.
	HeLa
p-MLC(Thr18/Ser19)2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-12896
	goat
	p	1%PFA, 4%PFA
	MDCK II
p-MYPT1 (T853)2011-10-28		WB	IF	IHC	IP
	Cell Signaling	1/1000
	#4563S
	rabbit
	m, h	TBS使用、BSAブロッキング Use TBS and BSA for blocking
	マウス非筋細胞
Phospho myosin light chain 2 (Ser19)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	3675S
	mouse
	p	1%PFA, 4%PFA
	MDCK II, EpH4, MTD-1A, L
Phospho myosin light chain 2 (Thr18/Ser19)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	3674L
	rabbit
	p	1%PFA, 4%PFA
	MDCK II, EpH4, MTD-1A, L
Phospho-MEK1/2 (Ser217/221)2013-09-12	41G9	WB	IF	IHC	IP
	Cell Signaling Technology	x1000
	#9154
	Rabbit monoclonal
	IgG
	HeLa
phospho-MLC2 (T18/S19)2011-10-28		WB	IF	IHC	IP
	Cell Signaling		1/500
	#3674S
	rabbit
	m	TBS使用 Use TBS
	マウス非筋細胞
phospho-MLC2 (T19)2011-10-28		WB	IF	IHC	IP
	Cell Signaling		1/500
	#3675S
	mouse
	m	TBS使用 Use TBS
	マウス非筋細胞
Phospho-p44/42 MAP kinase (pT202/pY204)2011-09-27		WB	IF	IHC	IP
	Cell Signaling		100
	9106S
	mouse
	monoclonal	4%PFA
	MDCK II
phosphorylated MAP1B2011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	1B6	3%PFA (in PBS)
	mouse
	m
	マウス脳
phosphorylated MAP1B2011-08-10		WB	IF	IHC	IP
	自家製 Homemade		x100
	1B6	3%PFA (in PBS)
	mouse
	m
	マウス脳

【n】

【N】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
beta NAP2014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610892	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

【n】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
cleaved Notch12011-08-14		WB	IF	IHC	IP
	Cell Signaling
	2421S
	rabbit
	p

goatαNotch12011-08-10	M-20)	WB	IF	IHC	IP
	Santa Cruz
	sc-6015
	goat
	p

Mouse CD112/Nectin-22011-09-27	clone 502-27	WB	IF	IHC	IP
	MBL		100
	D083-3
	rat
	m	1%PFA, 4%PFA, MeOH, TCA
	EpH4
Mouseαrat　nestin2011-08-10		WB	IF	IHC	IP
	BD Pharmingen		x100
	556309	3%PFA (in PBS)
	mouse
	m
	マウス脳
N-Cadherin2011-08-14		WB	IF	IHC	IP
	BD transduction		x100
	610921	3%PFA (in PBS)
	mouse
	m
	マウス神経細胞, MEF
Na+/K+ ATP ase2011-10-14	C464.6	WB	IF	IHC	IP
	Millipore/Upstate		x 300
	05-369	2％ PFA 0.5%Triton, RT 15min, methanol 固定可能 Methanol fixation also applicable
	mouse
	Mono、IgG1	【抗原の種、部位】rabbit whole protein
	MDCK cells

【N】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Na+K+ ATPase2015-01-13		WB	IF	IHC	IP
	Upstate biotechnology		x100
	05-369	3%PFA (in PBS)
	Mouse
	IgG1κ
	mouse kidney frozen section
Na+K+ ATPase2015-02-27		WB	IF	IHC	IP
	Upstate biotechnology		x100
	05-369	3%PFA (in PBS)
	Mouse
	IgG1κ
	mouse kidney frozen section

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Na,K-ATPase2011-11-26	a5	WB	IF	IHC	IP
	Developmental study hybridoma bank S
		4％PFA +0.1％Triton
	mouse
	IgG
	Drosophila nervous system
Na,K-ATPase2011-11-26	Nrv5F7	WB	IF	IHC	IP
	Developmental study hybridoma bank S
		4％PFA +0.1％Triton
	mouse
	IgG
	Drosophila nervous system
NaK ATPase α1subunit2012-04-04	α6F monoclonal	WB	IF	IHC	IP
	Developmental Studies Hybridoma Bank		x50
		3%PFA in PBS
	mouse	0.05% saponin
	IgG2a	concentrated culture sup
	Caco2	JBC 263, 4347-54, 1988
NaK ATPase αsubunit2012-04-04	α5 monoclonal	WB	IF	IHC	IP
	Developmental Studies Hybridoma Bank		x50
		3%PFA in PBS
	mouse	0.05% saponin
	IgG1	concentrated culture sup
	Caco2	EMBO J 8, 193-202
NDC802018-07-26	C-11	WB	IF	IHC	IP
	Santa Cruz Biotechnology		1/50
	sc-515550	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
Nectin 1 (Human)2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-28639
	rabbit
	p	1%PFA, 4%PFA, MeOH
	DLD-1
Nectin 2 (Human)2011-09-27		WB	IF	IHC	IP
	Santa Cruz		100
	sc-69719
	mouse
	m	MeOH, 1%PFA
	DLD-1
NEDD12018-07-26		WB	IF	IHC	IP
	abcam		1/1000
	ab57336	MeOH fixation
	Mouse	0.05% triton X-100

	HeLa
neomycin phosphotransferase II2011-08-10		WB	IF	IHC	IP
	Upstate	x1000
	06747MN
	rabbit
	p
	マウス脳
Nesprin-12011-10-13 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	X200	X100
	ab24742
	rabbit	PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton or MeOH
	IgG
	HeLa細胞
nestin2012-12-12		WB	IF	IHC	IP
	Chemicon			x500
	MAB353	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
		radial glial marker
	mouse brain
NeuN2012-12-12		WB	IF	IHC	IP
	Chemicon			x200
	MAB377	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG1	pan-neuron
	mouse brain
NeuroD2012-12-12		WB	IF	IHC	IP
	Santa Cruz			x100
	sc-1084	4%PFA (in PBS)
	goat	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG	young postmitotic neuron
	mouse brain
neurofilament2011-08-10	SMI-32	WB	IF	IHC	IP
	Covance		x100
	SMI32R	3%PFA (in PBS)
	mouse
	m
	マウス脳
neurofilament 2002011-08-10	NE14	WB	IF	IHC	IP
	Sigama		x100
	N5389	3%PFA (in PBS)
	mouse
	m
	マウス脳
Neurofilament 70k2012-12-12		WB	IF	IHC	IP
	Millipore			x200
	MAB1615SP	4%PFA (in PBS)
	mouse	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG1
	mouse brain
neurofilament,phosphorylated2011-08-10		WB	IF	IHC	IP
	Covance		x100
	SMI31R	3%PFA (in PBS)
	mouse

	マウス脳
neuroglian2011-11-26	BP104	WB	IF	IHC	IP
	Developmental study hybridoma bank S
		4％PFA +0.1％Triton
	mouse

	Drosophila nervous system
neuronal nuclei2011-08-10	NeuN	WB	IF	IHC	IP
	Chemicon		x100
	MAB377	3%PFA (in PBS)
	mouse
	m
	マウス脳
neurons2011-11-26	22C10	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton
	mouse
	IgG
	Drosophila nervous system
neurotactin2011-11-26	BP106	WB	IF	IHC	IP
	Developmental study hybridoma bank S
		4％PFA +0.1％Triton
	mouse

	Drosophila embryo
NGF-receptor　2011-08-10	p75	WB	IF	IHC	IP
	NeoMarkers		x100
	MS394	3%PFA (in PBS)
	mouse
	m
	マウス小腸
NGF-receptor　2011-08-12	p75	WB	IF	IHC	IP
	NeoMarkers

	mouse
	m	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

NMDAR2A2011-08-10		WB	IF	IHC	IP
	Millipore
	AB1555P	3%PFA (in PBS)
	rabbit
	p	IFＯＫ？
	マウス脳
nNOS2013-02-01		WB	IF	IHC	IP
	Cayman	x100
	160870
	rabbit (poly)


Notch extra2011-11-26	F461.3B	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton


	Drosophila embryo, discs, nervous system
Notch extra2011-11-26	C458.2H	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton
	mouse

	Drosophila embryo, discs, nervous system
Notch intra2011-11-26	C17.9C6	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton
	mouse

	Drosophila embryo, discs, nervous system
Notch intra2011-11-26	C17.9C6	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton
	mouse

	Drosophila embryo, discs, nervous system
NOV/CCN32012-12-12		WB	IF	IHC	IP
	R and D systems			x500
	AF1640	4%PFA (in PBS)
	goat	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG	adult CA1 pyramidal cell
	mouse brain
NSF2011-08-29		WB	IF	IHC	IP
	Trunsduction	x200

	mouse
	mono
	Hela細胞
NuMA2014-06-04		WB	IF	IHC	IP
	abcam		x1000
	ab36999	3% PFA (in PBS), 4%FA in PHEM
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, NCB 2012
NuMA2014-06-04		WB	IF	IHC	IP
	cell signaling		x100
	3888	3% PFA (in PBS), 4%FA in PHEM
	rabbit
	IgG
	HeLa	Kiyomitsu and Cheeseman, NCB 2012
NuMA2018-07-26		WB	IF	IHC	IP
	Cell Signaling Technology		1/1000
	3888S	MeOH fixation
	Rabbit	0.05% triton X-100

	HeLa
Nup1332014-07-11		WB	IF	IHC	IP
	Proteintech Group		x400
	12405-1-AP	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup1332014-08-01		WB	IF	IHC	IP
	Proteintech Group		x400
	12405-1-AP	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
Nup1532016-07-11	7A8	WB	IF	IHC	IP
	Progen	1/20	1/10
	651141	4% PFA/PBS
	Mouse
	IgG1
	PC12
Nup352014-08-01		WB	IF	IHC	IP
	SIGMA		x400
	HPA018401	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup352014-08-01		WB	IF	IHC	IP
	SIGMA		x400
	HPA018401	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
Nup622012-01-19	53, monoclonal mouse	WB	IF	IHC	IP
	BD Biosciences		X200-X500
	610497	PFA, Triton
	mouse
	IgG2b
	HeLa細胞
Nup622014-08-01		WB	IF	IHC	IP
	BD transduction		x400
	610497	4%PFA/PBS
	mouse
	IgG2b
	mouse oocyte (pronucleus)
Nup622014-08-01		WB	IF	IHC	IP
	BD transduction		x400
	610497	ice cold Methanol
	mouse
	IgG2b
	mouse oocyte (pronucleus)
Nup882012-01-19	22,monoclonal	WB	IF	IHC	IP
	BD Biosciences		X300
	611896	PFA, Triton
	mouse
	IgG1
	HeLa細胞
Nup982014-08-01		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2598	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup982014-08-01		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2598	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
rabbit Na+/K+ ATPase a-12011-08-10		WB	IF	IHC	IP
	Upstate		x100
	5369	3%PFA (in PBS)
	mouse
	m
	マウス小腸

【O】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
mouseαOccludin2011-08-10		WB	IF	IHC	IP
	invitrogen
	33-1500
	mouse
	m

OCRL12011-08-29		WB	IF	IHC	IP
	LIFESPAN		x100-

	rabbit	【PFA+Triton】:○,【PFA+Saponin】:△,【IS】:○,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
OCRL12011-08-29		WB	IF	IHC	IP
	Santa Cruz	ダメ?	ダメ

	goat	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
OCRL12011-08-29	4A6	WB	IF	IHC	IP
	Abnova	x500-	ダメ

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a
	Hela細胞
Oct3/42011-09-22		WB	IF	IHC	IP
	R&D systems		5 uG/ml
	MAB-1759
	rat	4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
	Mab
	mouse E3.5-6.5 embryo. TSC
Olig22012-12-11		WB	IF	IHC	IP
	R and D systems			x1000
	AF2418	4%PFA (in PBS)
	goat	PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
	IgG	Oligodendrocyte
	mouse brain
OPA1 (human 708-830aa)2011-10-07	18/OPA1	WB	IF	IHC	IP
	BD Transduction	500-2000	50
	No.612606	4%PFA/PBS,0.01%TritonX100
	mouse

	HeLa、MEF
Opsin2011-08-14		WB	IF	IHC	IP
	SIGMA
	O4886
	mouse
	m