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Antibody【Antigen:U-X】｜一般社団法人　日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody【Antigen:U-X】

【U】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
human Unc18-1/22011-08-10 N-19WBIF IHCIP
Santa Cruzx1000
sc14560
goat
p

マウス
human Unc18-22011-08-10 C-20WBIF IHCIP
Santa Cruzx1000
sc14563
goat
p

マウス
mouse ubiquilin2011-08-10 WBIF IHCIP
Calbiochem
662052
rabbit
p

試してない？
Not used
UBC92011-09-17 WBIF IHCIP
IMGENEX
IMG-3286
goat
Poly

Hela細胞
UBC92011-09-28 WBIF IHCIP
IMGENEXx1000
IMG-3286
goat
Poly

HeLa細胞
ubiquitin2011-09-27 FK2WBIF IHCIP
Enzo Life Science1:10001:2000
BML-PW8810-0500
mouse
m
Recognizes mono- and polyubiquitinylated conjugates. Species independent.
MEF, HeLa, A549, MCF7他
Ubiquitin2011-08-29 MAB1510WBIF IHCIP
Chemiconx500
mouse
Mono, IgG1
Before WB, autoclave the membrane
Hela細胞
ubiquitin2011-08-10 WBIF IHCIP
Dako
Z0458
rabbit
p

試してない？
Not used
Ubiquitylated Proteins2011-08-29 clone FKIIWBIF IHCIP
日本バイオテスト研究所 Nippon Bio-test Laboratoriesx1000
mouse
Mono, IgG1
Permeabilize before fixation
Hela細胞
Ubiqutin2011-10-13 WBIF IHCIP
CHEMICONX200
MAB1510
mouse
PFA+Triton
Mono, IgG1

HT1080細胞
ULK12011-09-27 H-240WBIF IHCIP
Santa Cruz1:1000
sc-33182
rabbit
p

utrophin2011-10-14 ―WBIF IHCIP
Santa Cruzx 1,000x 1,000
UT-2
2％ PFA 0.5%Triton, RT 17min
rabbit
Poly
【抗原の種、部位】human 1768-2078 a.a.
MDCK cells

【V】

【v】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Endobrevin / VAMP82012-11-13 WBIF IHCIP
Synaptic Systemsx1000x100
104302
3%PFA (in PB)
Rabbit
acinar細胞のzymogen顆粒が強く染まる。WBはケラチンのノンスペが多い
Zymogen granules are strongly stained in IF, but nonspecific keratin staining is evident in WB.
マウス膵臓
human Villin2011-08-10 C-19WBIF IHCIP
Santa Cruzx100
sc7672
3%PFA (in PBS)
goat
p
染色薄い
Weak staining
マウス小腸
human Vimentin2011-08-10 WBIF IHCIP
Chemicon
CBL202
mouse
m

mVAMP72011-08-10 0H307WBIF IHCIP
IBL
2930
rabbit

rabbitαVAMP82011-08-10 WBIF IHCIP
SYSYx1000
104302
rabbit
p

マウス
Synaptobrevin / VAMP22012-11-13 69.1 monoclonalWBIF IHCIP
Synaptic Systemsx1000x100
104211
3%PFA (in PB)
mouse
IgG1
β細胞のinsulin顆粒は強く染まり、acinar細胞のzymogen顆粒は弱い
Insulin granules are strongly stained while zymogen granules are weakly stained.
マウス膵臓
synaptobrevin/VAMP 22012-08-03 WBIF IHCIP
Synaptic Syx100
104211
3%PFA in PBS
mouse
0.05% saponin

PC12
TI-VAMP protein2011-08-10 VAMP 7WBIF IHCIP
Novus Bx100
NB1002325
3%PFA (in PBS), methanol
mouse
m

マウス小腸
TI-VAMP protein2011-08-10 VAMP 7WBIF IHCIP
Novus Bx100
NB1002325
3%PFA (in PBS), methanol
mouse
m

マウス小腸
VAMP3 (cellubrevin)2015-06-02 WBIF IHCIP
synaptic systemsx1000x100
104102
3%PFA (in PBS)
rabbit
For WB, 20ug of neural progenitor cell lysate was used
mouse primary neuron, neural progenitor cell
VAMP42011-08-10 WBIF IHCIP
affinity bioreagents
pa1-768
rabbit
p

VAMP42011-08-29 WBIF IHCIP
ABRx500-
rabbit
【PFA+Triton】:×,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
Poly

Hela細胞

【V】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
VAMP42016-09-08 WBIF IHCIP
Synaptic systems1/10001/500
136002
4% PFA
Rabbit
IgG

HeLa, PC12

【v】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
VAMP72011-08-10 WBIF IHCIP
Abnovax100
PAB5936
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
VAMP82011-08-29 WBIF IHCIP
SySy
rabbit
Poly

Hela細胞
Vasa(Drosophila）2011-11-26 d-260WBIF IHCIP
Santa Cruzx50
sc30210
4％PFA +0.1％Triton
rabbit

Drosophila germline
VE cadherin2011-10-28 WBIF IHCIP
Santa Cruz1/200
sc6458
95%EtOH/Acetone
goat
m

マウス組織染色可
Vimentin2011-08-29 WBIF IHCIP
SySyダメ
rabbit
【PFA+Triton】:×【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
Poly

Hela細胞

【V】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Vimentin (Chicken)2016-08-10 AMF-17bWBIF IHCIP
Developmental Studies Hybridoma Bank (DSHB)x300
4%PFA (in phosphate buffer)
Mouse
0.1% TritonX-100/PBS
IgG1

MDCK cell
Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Vinculin2011-09-27 clone VIN 11-5 WBIF IHCIP
Sigma100
V4505
mouse
m
1%PFA, MeOH > TCA > 4%PFA
試した限り全ての培養細胞
vinculin2011-10-14 hVIN-1WBIF IHCIP
Sigmax 500
V9131
2％ PFA 0.5%Triton, RT 15min,
methanol 固定可能
Methanol fixation also applicable
mouse
Mono、IgG1
【抗原の種、部位】human whole protein
MDCK cells
Vinculin2011-10-13
About Product
MonoclonalWBIF IHCIP
AbcamX500X200
ab11194
mouse
PFA+Triton or MeOH
IgG1

HeLa細胞、TIG-1細胞
Vma2 (V-ATPase B subunit)2011-08-31 IgG1WBIF IHCIP
Invitrogen (Molecular Probes)
A-6427
Roberts et al. (1991) Methods Enzymol. Vol 194, 644-661
Mouse
monoclonal

Vps102011-08-31 IgG2aWBIF IHCIP
Invitrogen (Molecular Probes)
A-21274
Mouse
monoclonal

VSVG2011-08-29 P5D4WBIF IHCIP
SIGMA
V5507
mouse
Mono, IgG1

Hela細胞
Vti1a2011-08-29 clone 45WBIF IHCIP
BD Transductionx200-
mouse
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
Mono, IgG1

Hela細胞
Vti1a2011-08-29 WBIF IHCIP
SySy
rabbit
Poly

Hela細胞
Vti1a2012-01-25 WBIF IHCIP
BD Transductionx100
V85620
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
Vti1a2012-05-21 WBIF IHCIP
BD Transduction Labx100
V85620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
Caco2
Vti1a2012-05-21 WBIF IHCIP
BD Transduction Labx100
V85620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Golgi sampler kit (BD Transduction Lab)
MDCK
Vti1b2011-08-29 clone 7WBIF IHCIP
BD Transductionx100-
mouse
【PFA+Triton】:○,【PFA+Saponin】:○,【MeOH】:◎,【MeOH】:◎【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
Mono, IgG1

Hela細胞
Vti1b2011-08-29 WBIF IHCIP
SySy
rabbit
Poly

Hela細胞

【X】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
XBP12011-09-17 WBIF IHCIP
Santa Cruz
#sc-7160
rabbit
Poly

Hela細胞
XBP12011-09-28 WBIF IHCIP
Santa Cruzx1000
#sc-7160
rabbit
Poly

HeLa細胞
Xpress2011-08-29 WBIF IHCIP
Invitrogenx5000x1000x800
mouse
Mono, IgG1

Hela細胞

【W】

【w】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Wg2011-11-26 4D4WBIF IHCIP
Developmental study hybridoma bank S
4％PFA +0.1％Triton
mouse
IgG

Drosophila embryo, discs, nervous system

【W】

Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Wingless2014-09-04 4D4WBIF IHCIP
DHSBx100
4%PFA (in phosphate buffer)
mouse
IgG1

Drosohila imaginal disc
Antigen
last update		Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
WIPI22014-06-30 polyclonalWBIF IHCIP
Sigma-Aldrichx100-200
SAB4200400
4%PFA (in PBS)
rabbit
WBでも使用できるはず
MEF, HeLa

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