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Antibody(neuroscience)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(neuroscience)

【A】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho-Akt(Ser473)2011-10-03 587F11WBIF IHCIP
Cell signalingx200
mouse
Mono, IgG2b

HEK293T細胞,rat hippocampal neurons
axons, CNS2011-11-26 BP102WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo, nervous system
phospho-Akt2011-10-03 WBIF IHCIP
cell signalingx200
#9277
rabbit
【Formalin in PBS+0.05%Triton】:○
poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
APPc2011-08-10 Amyloidb1-40 1-42WBIF IHCIP
IBLx1000
18580/582?
rabbit

GABA2011-08-10 g-aminobutyric acidWBIF IHCIP
Sigmax100
A2052
3%PFA (in PBS)
rabbit
IFではよくわからないが、ImEMではシナプス小胞が染まっているように見える
By ImEM, synaptic vesicles can be stained by this Ab, but staining is not evident by IF.
マウス脳
ALDH1L12012-12-11 WBIF IHCIP
abcamx500
ab87117
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
astrocyte
mouse brain
actin2011-10-03 C4WBIF IHCIP
Chemiconx1000?3000
MAB1501R
mouse
Mono, IgG1k

HEK293T細胞、rat hippocampal neurons
amyloid b protein2011-08-10 WBIF IHCIP
Chemiconx100
MAB1560
3%PFA (in PBS)
mouse
m

neuro2A細胞
mouse pan-AMPAR2011-08-10 WBIF IHCIP
Frontier sci.x100
panAMPAR-GPAf5801
3%PFA (in PBS)パパインの前処理必要
Pretreatment by papain is required
guinea pig
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
APPL(β-amyloid precursor protein)(Drosophila)2011-11-26 dR-14WBIF IHCIP
Santa Cruzx50
sc-26715
4%PFA +0.1%Triton
goat IgG
IgG

Drosophila discs, nervous system

【B】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Bridge of sevenless(dH-18)2011-11-26 sc-15818WBIF IHCIP
Santa Cruzx10
sc-15818
4%PFA +0.1%Triton
Goat poly

Drosophila eye

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
chp2011-11-26 24B10WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse
IgG

Drosophila eye
cut2011-11-26 2B10WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
Cortactin2011-10-03 4F11WBIF IHCIP
Milliporex1000x100?500
05-180
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,
【Formalin in Ringer液+Methanol】:○,
Mono,IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
calbindin2012-12-11 WBIF IHCIP
SWANTx500
300
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
mature DG granule cell, adult CA1 pyramidal cell, a type of interneuron
mouse brain
ChromograninB2011-08-14 WBIF IHCIP
BD transduction
611646
mouse
m

calretinin2012-12-11 WBIF IHCIP
Chemiconx2000
AB1550
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
immature DG granule cell, a type of interneuron, Cajal-retzius cell
mouse brain
calbindin D-28K2011-08-10 WBIF IHCIP
Chemiconx100
AB1778
3%PFA (in PBS)
rabbit
p

マウス小脳
calbindin2012-12-11 WBIF IHCIP
Chemiconx1000x500
AB1778
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
mature DG granule cell, adult CA1 pyramidal cell, a type of interneuron
mouse brain, primary hippocampal neuron
calretinin2012-12-11 WBIF IHCIP
Chemiconx2000
AB5054
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
immature DG granule cell, a type of interneuron, Cajal-retzius cell
mouse brain
CNPase2012-12-11 WBIF IHCIP
Chemiconx500
MAB326
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
oligodendrocyte
mouse brain
CaMKII2012-12-11 WBIF IHCIP
Chemiconx1000
MAB8699
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min

mouse brain

【c】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Cux1 (CDP)2014-05-09 WBIF IHCIP
santa cruz biotechnologyx50
sc-13024
3%PFA (in phosphate buffer)
rabbit
pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
mouse cerebrum, cerebellum

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
calbindin2012-12-11 WBIF IHCIP
Santa Cruzx500
sc-7691
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
mature DG granule cell, adult CA1 pyramidal cell, a type of interneuron
mouse brain

【c】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
calbindin D28K (C-20)2014-05-09 WBIF IHCIP
santa cruz biotechnologyx50
sc-7691
3%PFA (in phosphate buffer)
goat
pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
mouse cerebrum, cerebellum

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
rat chromogranin A2011-08-10 WBIF IHCIP
Yanaiharax100
Y291
3%PFA (in PBS)
rabbit
neuroendocrine cellsが染まる
This Ab stains neuroendocrine cells.
マウス小腸

【D】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
phospho DARPP-322011-08-14 Thr34WBIF IHCIP
Cell Signaling
2304S
rabbit
p

2本ある
dopamine2011-08-10 WBIF IHCIP
Chemicon
AB122S
rabbit
p

doublecortin (DCX)2012-12-11 WBIF IHCIP
Chemiconx200
AB2253
4%PFA (in PBS)
guinea pig
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
serum
immature neuron
mouse brain
dopamine beta hydroxylase2011-08-10 DBHWBIF IHCIP
Chemicon
MAB308
mouse
m

dopamine transporter2011-08-10 DTWBIF IHCIP
millipore
MAB369
rat
m

試してない?
Not used

【E】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Elav2011-11-26 7E8A10WBIF IHCIP
Developmental study hybridoma bank Sx4000
4%PFA +0.1%Triton
rat
IgG

Drosophila embryo, discs, nervous system
Elav2011-11-26 9F8A9WBIF IHCIP
Developmental study hybridoma bank Sx4000
4%PFA +0.1%Triton
mouse
IgG

Drosophila embryo, discs, nervous system
En2011-11-26 4D9WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
eyes shut2011-11-26 21A6WBIF IHCIP
Developmental study hybridoma bank Sx10
4%PFA +0.1%Triton
mouse
IgG

Drosophila pupal eye
ELAV2014-11-12 7E8A10WBIF IHCIP
DSHBx100
4%PFA (in phosphate buffer)
rat
IgG2a

Drosohila imaginal disc
ELAV2014-11-12 9F8A9WBIF IHCIP
DHSBx20
4%PFA (in phosphate buffer)
mouse
IgG1

Drosohila imaginal disc
Elavl2(HuB)2012-12-11 WBIF IHCIP
GENE TEXx1000x500
GTX12030
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
a type of pyramidal neuron
mouse brain, primary hippocampal neuron

【F】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Fas I 2011-11-26 F5H7WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
Fas III2011-11-26 7G10WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse
IgG

Drosophila embryo, discs, nervous system
Fas II2011-11-26 1D4WBIF IHCIP
Developmental study hybridoma bank Sx10
4%PFA +0.1%Triton
mouse IgG1
IgG1

Drosophila embryo, discs, nervous system
FOXP12012-12-11 WBIF IHCIP
abcamx200
ab16645
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
CA1 pyramidal cell
mouse brain
Flag M22011-10-03 WBIF IHCIP
Sigmax1000?3000x1000
F3165
mouse
Mono,IgG1

HEK293T細胞,COS7細胞,rat hippocampal neurons

【G】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
GFP2011-10-03 WBIF IHCIP
MBLx1000
rabbit
Poly
メーカー推奨:WB x5000~10000
HEK293T細胞、rat hippocampal neurons
Giantin2011-10-03 WBIF IHCIP
covancex1000
A488-114L
rabbit
【Formalin in PBS+0.05%Triton】:○
Poly
Alexa Fluor 488 Labeled
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
GFAP2012-12-11 G-A-5WBIF IHCIP
Sigmax500
G3893
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
astrocyte, adult neural stem cell
mouse brain
GAD672011-10-03 WBIF IHCIP
Chemicon (Millipore)x20000x1000
MAB5406
mouse
【Formalin in Ringer液+0.05%Triton】:○
Mono, IgG2a
メーカー推奨:WB x5000~10000
ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
mouse Ganglionic eminence neurons

【H】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
HRP2011-11-26 WBIF IHCIP
Jackson Immunoresearch Lab.x2000
323-005-021
4%PFA +0.1%Triton
rabbit
IgG

Drosophila embryo, discs, nervous system
Homer32012-12-11 WBIF IHCIP
Cosmo biox500
H104-03
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
postsynaptic dendite(CA3)
mouse brain

【I】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Iba12012-12-11 WBIF IHCIP
abcamx500
ab5076
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
microglia
mouse brain

【J】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
JIP12011-10-03 WBIF IHCIP
Santa Cruz×400 ×5000
rabbit
【Formalin in PBS+0.05%Triton】:○
Poly,M-300 sc-15353

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons

【L】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
L12011-10-03 C20WBIF IHCIP
Santa cruzx1000
goat
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
L12011-08-10 8D9WBIF IHCIP
DSHBx100
8D9
3%PFA (in PBS)
mouse

マウス脳
L12011-08-12 8D9WBIF IHCIP
DSHB
8D9
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
L12011-08-10 WBIF IHCIP
Chemiconx100
MAB5272
3%PFA (in PBS)
rat
m

マウス脳
L12011-08-12 WBIF IHCIP
Chemicon
MAB5272
rat
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

【M】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
myc2011-10-03 PL14WBIF IHCIP
MBLx1000
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Mono,IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
COS細胞,rat hippocampal neurons
Mash12012-12-12 WBIF IHCIP
BD pharmingenx100
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
proneural progenitors
mouse brain
phosphorylated MAP1B2011-08-10 WBIF IHCIP
自家製 Homemadex100
1B6
3%PFA (in PBS)
mouse
m

マウス脳
mouse MAP22011-08-10 WBIF IHCIP
自家製 Homemadex100
2G5
3%PFA (in PBS)
mouse
m

マウス脳
myc2011-10-03 WBIF IHCIP
MBLx1000x1000
562-5
rabbit
Poly

HEK293T細胞,COS細胞,rat hippocampal neurons
Math22012-12-12 WBIF IHCIP
Chemiconx500
AB9240
4%PFA (in PBS)
rabbit
0.1% Triton
IgG
pyramidal cell
primary hippocampal neuron
MAP22011-08-10 clone: HM-2WBIF IHCIP
Sigmax100
M4403
3%PFA (in PBS)
mouse
m

マウス脳
MAP22011-10-03
About Product
HM-2, monoclonalWBIF IHCIP
Sigma-AldrichX5000
M4403
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
MAP22012-12-12 WBIF IHCIP
Sigmax500
M4403
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
dendrites
mouse brain
mouse MAP 2a,b,c Ab-32011-08-10 WBIF IHCIP
NeoMarkersx100
MA250
3%PFA (in PBS)
mouse
m

マウス脳
mouse metabtropic GluR1a2011-08-10 WBIF IHCIP
Frontierx100
mGluR1aGoAf12201
3%PFA (in PBS)
goat
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
mouse metabtropic GluR1a2011-08-10 WBIF IHCIP
Frontierx100
mGluR1aGoAf12201
3%PFA (in PBS)
goat
p
OK(北大渡辺先生作)パパインによる前処理必要
Made by Prof M. Watanabe (Hokkaido Univ.) pretreatment by papain is required.
マウス脳
Math2 (NeuroD6)2012-12-12 WBIF IHCIP
Santa Cruzx100
sc-19251
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
pyramidal cell
mouse brain

【N】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
NGF-receptor 2011-08-12 p75WBIF IHCIP
NeoMarkers
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
neuroglian2011-11-26 BP104WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila nervous system
neurons2011-11-26 22C10WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse
IgG

Drosophila nervous system
neurotactin2011-11-26 BP106WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo
nNOS2013-02-01 WBIF IHCIP
Caymanx100
160870
rabbit (poly)

Mouseαrat nestin2011-08-10 WBIF IHCIP
BD Pharmingenx100
556309
3%PFA (in PBS)
mouse
m

マウス脳
NMDAR2A2011-08-10 WBIF IHCIP
Millipore
AB1555P
3%PFA (in PBS)
rabbit
p

IFOK?
マウス脳
NOV/CCN32012-12-12 WBIF IHCIP
R and D systemsx500
AF1640
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
adult CA1 pyramidal cell
mouse brain
Neurofilament 70k2012-12-12 WBIF IHCIP
Milliporex200
MAB1615SP
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1

mouse brain
nestin2012-12-12 WBIF IHCIP
Chemiconx500
MAB353
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
radial glial marker
mouse brain
neuronal nuclei2011-08-10 NeuNWBIF IHCIP
Chemiconx100
MAB377
3%PFA (in PBS)
mouse
m

マウス脳
NeuN2012-12-12 WBIF IHCIP
Chemiconx200
MAB377
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
pan-neuron
mouse brain
NGF-receptor 2011-08-10 p75WBIF IHCIP
NeoMarkersx100
MS394
3%PFA (in PBS)
mouse
m

マウス小腸
neurofilament 2002011-08-10 NE14WBIF IHCIP
Sigamax100
N5389
3%PFA (in PBS)
mouse
m

マウス脳
NeuroD2012-12-12 WBIF IHCIP
Santa Cruzx100
sc-1084
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
young postmitotic neuron
mouse brain
neurofilament,phosphorylated2011-08-10 WBIF IHCIP
Covancex100
SMI31R
3%PFA (in PBS)
mouse

マウス脳
neurofilament2011-08-10 SMI-32WBIF IHCIP
Covancex100
SMI32R
3%PFA (in PBS)
mouse
m

マウス脳

【O】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Olig22012-12-11 WBIF IHCIP
R and D systemsx1000
AF2418
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
Oligodendrocyte
mouse brain
Opsin2011-08-14 WBIF IHCIP
SIGMA
O4886
mouse
m

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
prospero2011-11-26 MR1AWBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse

Drosophila nervous system
Prox12012-12-12 WBIF IHCIP
abcamx2000x1000
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain, primary hippocampal neuron
PI3 kinase p852011-10-03 WBIF IHCIP
upstatex1000
06-497
rabbit
poly

rat hippocampal neurons
Par32011-10-03 WBIF IHCIP
upstatex500
07-330
rabbit
【Formalin in PBS+0.05%Triton】:○
poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Prox12012-12-12 WBIF IHCIP
Upstatex1000
07-537
rabbit
RIPA
IgG
DG, svz in GE, interneuron
mouse brain lysate
Prox12012-12-12 WBIF IHCIP
RELIA Tech GmbHx1000
102-PA32AG
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain
PDGFreceptor alpha chain2012-12-12 WBIF IHCIP
BD pharmingenx500
558774
4%PFA (in PBS)
rat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG2ak
Oligodendrocyte
mouse brain
PI3 kinase p852011-10-03 4WBIF IHCIP
BDx500
610045
mouse
Mono, IgG2a

rat hippocampal neurons
Pax62012-12-12 WBIF IHCIP
Chemiconx200
AB5409
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
dorsal neural progenitors
mouse brain
Prox12012-12-12 WBIF IHCIP
Chemiconx500
AB5475
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain
Prox12012-12-12 WBIF IHCIP
R and D systemsx2000x1000
AF2727
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain, primary hippocampal neuron
human p75 pAb2011-08-10 WBIF IHCIP
Promegax100
G3231
3%PFA (in PBS)
rabbit
神経らしき線維が染まる
Axon-like fibers are detected by this Ab.
マウス小腸
Parvalbumin2012-12-12 WBIF IHCIP
Chemiconx100
mab1572
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
a type of interneuron, centrosome?
mouse brain
PSA-NCAM2012-12-12 WBIF IHCIP
Chemiconx200
MAB5324
ice Methaol
mouse
IgM
immature neuron
mouse brain
Prox12012-12-12 4G10WBIF IHCIP
Chemiconx1000
MAB5654
mouse
RIPA
IgG2bk
DG, svz in GE, interneuron
mouse brain lysate
Parvalbumin2012-12-12 WBIF IHCIP
Sigmax100
P3088
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
a type of interneuron, centrosome?
mouse brain
Prox12012-12-12 WBIF IHCIP
Covancex500
PRB-238C
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG, svz in GE, interneuron
mouse brain

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Repo2011-11-26 8D12WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila nervous system
Rhodopsin2011-11-26 4C5WBIF IHCIP
Developmental study hybridoma bank Cx15000x2000
4%PFA +0.1%Triton
mouse
IgG

Drosophila eye
rab33a2011-10-03 8A11WBIF IHCIP
Abnovax1000
H00009396-M04
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer+0.05%Triton】:○
Mono,IgG2ak

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Synaptophysin2011-10-03 WBIF IHCIP
Progenx1000x2500
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Mono,

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
slit2011-11-26 C55S.6DWBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptophysin 12016-10-18 7.2WBIF IHCIP
Synaptic systems1/10001/500
101011
4% PFA
Mouse
IgG

primary neuron, PC12

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptobrevin/VAMP 22011-08-10 WBIF IHCIP
Synaptic Sy
104211
mouse
m

synaptobrevin/VAMP 22011-08-12 WBIF IHCIP
Synaptic Sy
104211
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
synaptobrevin/VAMP 22011-08-10 WBIF IHCIP
Synaptic Syx1000
104211
3%PFA (in PBS)
mouse
m

マウス

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptobrevin 2 (VAMP2)2016-08-10 69.1WBIF IHCIP
Synaptic systems1/10001/500
104211
4% PFA
Mouse
IgG

primary neuron, PC12
synaptotagmin 12016-10-18 41.1WBIF IHCIP
Synaptic systems1/10001/500
105011
4% PFA
Mouse
IgG

primary neuron, PC12
syntaxin 12016-09-08 78.2WBIF IHCIP
Synaptic systems1/10001/500
110011
4% PFA
Mouse
IgG

primary neuron, PC12

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Serotonin, 5-HT2019-09-10 WBIF IHCIP
ImmunoStarx500-1000
20080
Parrafin section
Rabbit
IgG
Antigen retrieved with Immunosaver (Nisshin EM)
マウス小腸
S100beta2012-12-12 EP1576YWBIF IHCIP
abcamx200
ab52642
4%PFA (in PBS)
rabbit mo
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
mature astrocyte
mouse brain
somatostatin2012-12-12 WBIF IHCIP
Chemiconx100
AB5494
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
a type of interneuron
mouse brain
Sox22012-12-12 WBIF IHCIP
Chemiconx100
AB5603
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
progenitor, glia
mouse brain
human SNAP-252011-08-12 WBIF IHCIP
Chemicon
AB5871P
goat
p
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
human SNAP-252011-08-10 WBIF IHCIP
Chemicon
AB5871P
goat
p

synapsin I2012-12-12 WBIF IHCIP
abcamx500
ab8
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
synapse
mouse brain
STXBP62012-12-12 WBIF IHCIP
Sigma ATLASx200
HPA003552
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
DG granule cell
mouse brain
human synaptobrevin2011-08-10 VAMPWBIF IHCIP
Chemicon
MAB335
mouse
m

human synaptobrevin2011-08-10 VAMPWBIF IHCIP
Chemicon
MAB335
mouse
m

synaptophysin2012-12-12 WBIF IHCIP
Chemiconx100
MAB368
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG1
synapse
mouse brain
bovine synaptophysin2011-08-10 WBIF IHCIP
Chemicon
MAB5258
mouse
m

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
synaptophysin/p382014-05-09 WBIF IHCIP
Millipore (Roche)x1000
MAB5258
3%PFA (in phosphate buffer)
mouse
pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
mouse cerebrum, cerebellum

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma5000
S0664
mouse

pancreas lysate
syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma100
S0664
3%PFA (in PBS)
mouse

mouse primary neuron, neural progenitor cell, pancreas
Sox12012-12-12 WBIF IHCIP
Santa Cruzx100
sc-17318
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
progenitor, glia
mouse brain
Sox22012-12-12 WBIF IHCIP
Santa Cruzx100
sc-17320
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
progenitor, glia
mouse brain
SV22011-08-12 WBIF IHCIP
DSHB
SV2
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
SV22011-08-10 WBIF IHCIP
DSHB
SV2
mouse

SV22012-08-03 WBIF IHCIP
DSHBx100
SV2
3%PFA in PBS
mouse
0.05% saponin

PC12

【T】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
α-tubulin2011-10-03 WBIF IHCIP
Cell Signaling×250
2144
rabbit
【Formalin in PBS+0.05%Triton】:○
Poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Tyrosine hydroxylase (TH)2012-12-12 WBIF IHCIP
Chemiconx500
AB152
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
OB periglomerular neuron
mouse brain
rabbitαTbr12011-08-10 WBIF IHCIP
CHEMICONx100
ab9616
3%PFA (in PBS)
rabbit

マウス脳

【t】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Tbr12014-05-09 WBIF IHCIP
Milliporex500
AB9616
3%PFA (in phosphate buffer)
rabbit
pretreatment with 5%normal donkey serum, 0.1% TritonX-100 in PBS RT for 1hr before 1st antibody is recommended
mouse cerebrum, cerebellum

【T】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
mouseαTyrosine Hydroxylase2011-08-10 WBIF IHCIP
milliporex100
MAB318
3%PFA (in PBS)
mouse
m

マウス脳
Tau12011-08-14 WBIF IHCIP
CHEMICONx1000x100
MAB3420
3%PFA (in PBS)
mouse
m

マウス脳
bovine tau-12011-08-10 WBIF IHCIP
Chemiconx100
MAB3420
3%PFA (in PBS)
mouse
m

マウス脳
Tau-12011-10-03 PC1C6WBIF IHCIP
Chemiconx500
MAB3420
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,
Mono, IgG2a

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
mouseαNeuronal classαTubulin2011-08-10 WBIF IHCIP
Covancex100
mms-435p
3%PFA (in PBS)
mouse
m

マウス脳
beta 3-tubulin2011-10-03 Tuj1WBIF IHCIP
CovanceX2000
MMS-435P
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in PBS+Methanol】:○,
Mono, IgG2a

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
γ-Tubulin2011-10-03 GTU-88WBIF IHCIP
sigmax1000x500
T6557
mouse
【Formalin in PBS+0.05%Triton】:○
Mono, IgG1

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Acetylated tubulin2011-10-03 6-11B-1WBIF IHCIP
sigma×50000
T7451
mouse
【Formalin in PBS+0.05%Triton】:○
Mono

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Tubulin, Tyrosine2011-10-03 TUB-1A2WBIF IHCIP
Sigma×1500
T9028
mouse
【Formalin in PBS+0.05%Triton】:○
Mono,IgG3

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons

【V】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
VAMP3 (cellubrevin)2015-06-02 WBIF IHCIP
synaptic systemsx1000x100
104102
3%PFA (in PBS)
rabbit
For WB, 20ug of neural progenitor cell lysate was used
mouse primary neuron, neural progenitor cell
synaptobrevin/VAMP 22012-08-03 WBIF IHCIP
Synaptic Syx100
104211
3%PFA in PBS
mouse
0.05% saponin

PC12

【Z】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ZnT32012-12-12 WBIF IHCIP
Synaptic systemsx500
197-002
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
mossy fiber buttons
mouse brain
Zbtb202012-12-12 WBIF IHCIP
Sigma ATLASx100
HPA016815
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
Hippocampal neurons, astrocyte
mouse brain