Visualization of ER-to-Golgi trafficking of procollagen X (jst.go.jp)

This paper visualized the ER-to-Golgi transport of procollagen X, which is involved in cartilage remodeling, and analyzed the proteins required for its transport.

Deep learning-based segmentation of subcellular organelles in high-resolution phase-contrast images (jst.go.jp)

This paper shows the development of deep learning-based segmentation models for organelles in high-resolution phase-contrast images.

Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging (jst.go.jp)

This paper visualized the distribution of glycosyltransferases within the Golgi of mammalian cells and explored the determinants of their sub-Golgi localization.

Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells

This paper shows development of in vitro nuclear envelope reconstitution system using semi-intact cells.

A high-resolution phase-contrast microscopy system for label-free imaging in living cells

This paper shows a tuned-up phase-contrast microscopy system for visualizing dynamic organelle behaviors.

In vivo evidence for homeostatic regulation of ribosomal protein levels in Drosophila

Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease

This paper shows that the lysosomal membranes of cells derived from patients with Gaucher disease are susceptible to damage.

Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium

This paper shows that Coronin-1, which is an actin regulator, promotes cell rearrangement.

Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition

This paper shows that the activation of p38MAPK enhances CRP2 expression and induces phenotypic transition into myofibroblasts and that CRP2BP promotes transcription via the SRF/CArG-box as an adapter factor.

Visual quantification of prostaglandin E₂ discharge from a single cell

This paper shows the amount of prostaglandin E2 secreted from a single cell using a combination of imaging and simulation.

Rab32 and Rab38 maintain bone homeostasis by regulating intracellular traffic in osteoclasts

This paper shows that Rab32 and Rab38 are coordinately responsible for membrane trafficking that supports bone resorption in mouse osteoclasts.

Signal sequence-triage is activated by translocon obstruction sensed by an ER stress sensor IRE1α

This paper reports that translocon obstruction activates signal sequence-triage and the ER stress sensor IRE1α.

VAMP5 and distinct sets of cognate Q-SNAREs mediate exosome release

This paper shows that a novel MVB-localized SNARE VAMP5 regulates exosome release through the MVB-plasma membrane fusion.

FilGAP, a GAP for Rac1, down-regulates invadopodia formation in breast cancer cells

This paper shows that the RacGAP protein FilGAP is a negative regulator of invadopodia formation in breast cancer cells.

Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis

This paper shows sphingomyelin is an essential regulator of Ras excitability on the cell membrane that ensures efficient chemotaxis.

Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression

In this study, we have developed two fluorescent proteins of our own making to create Fucci5, which is free from license-related problems, in hopes of popularizing the Fucci technology in industry.

Proteomic Analysis of Fatty Liver Induced by Starvation of Medaka Fish Larvae

This paper shows the mechanism of starvation-induced development of fatty liver in medaka fish larvae analyzed by proteomic analysis.

Zebrafish imaging reveals hidden oncogenic-normal cell communication during primary tumorigenesis

This review introduces the cooperative and competitive communication between oncogenic cells and neighboring cells, as revealed by our recent zebrafish imaging studies.

Role of CRP2-MRTF interaction in functions of myofibroblasts

This paper shows that CRP2 plays a significant role in the functional expression of myofibroblasts by direct interaction between CRP2 and MRTF and reveals the detailed mechanism for CRP2-MRTF interaction.

Cell biology of protein-lipid conjugation

This review provides an overview of diverse forms of protein–lipid conjugation and summarizes the catalytic mechanisms and roles of these modifications.

A conserved WXXE motif is an apical delivery determinant of ABC transporter C subfamily isoforms

This paper shows a stretch of four amino acid, WXXE motif, as the apical expression determinant of the 12-TM configuration ABCC subfamily members in polarized epithelial cells.

A non-nucleotide agonist that binds covalently to cysteine residues of STING

This paper shows the identification of a novel STING agonist PAO, which does not target the cGAMP-binding pocket.

Suppression of P-cadherin expression as a key regulatory element for embryonic stem cell stemness

This paper shows that spontaneously extruded syntaxin4 induces the expression of P-cadherin protein in 2D-ES cells, leading to the loss of ES cell stemness.

Enhancement of Vivid-based Photo-Activatable Gal4 Transcription Factor in Mammalian Cells

This paper shows enhancement of Vivid-based blue light-activatable Gal4 transcription factor in mammalian cells to improve efficacy and reliability of optogenetic regulation of gene expressions.

Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast

This paper shows requirement of phosphatidylserine in the localization of plasma membrane proteins such as receptors.

Reconstitution of functional tight junctions with individual claudin subtypes in epithelial cells

This paper shows that claudin-deficient MDCK cells can be used to reconstitute tight junctions and analyze their function by single subtypes of the claudin family.