Capturing CDKs in Action: Live-Cell Biosensors Pioneer the New Frontiers in Cell Cycle Research

This paper comprehensively reviews the fluorescent sensors that visualize cell cycle regulatory CDK activities in real-time within living cells.

Tango1L but not Tango1S, Tali and cTAGE5 is required for export of type II collagen in medaka fish

This paper shows that only Tango1L is required for enlargement of COPII vesicles among 2 genes (2 splicing variants each) in medaka fish.

Live imaging of paracrine signaling: Advances in visualization and tracking techniques

This paper provides an overview of methods for visualizing paracrine signaling, which is a major mechanism of cell-to-cell communication.

The Role of Primary Cilia in Myoblast Proliferation and Cell Cycle Regulation during Myogenesis

This paper reviews the role of cilia in the myogenesis process, particularly in controlling the balance between myoblast proliferation and differentiation, and progress in imaging techniques for their analysis.

Impact of physiological ionic strength and crowding on kinesin-1 motility

This paper shows that ionic strength and molecular crowding close to intracellular levels maintain high kinesin motility.

Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles

This paper shows that internalized magnetic nanoparticles in cancer cells enable magnetic control of membrane damage in early endosomes.

A sensitive ERK fluorescent probe reveals the significance of minimal EGF-induced transcription

This paper shows a sensitive ERK activity probe, EKAREN5-gl, which can detect low-dose EGF-induced activity.

Multi-color fluorescence live-cell imaging in Dictyostelium discoideum

This paper describes development of 5-color live fluorescence imaging in Dictyostelium discoideum.

Possible roles of CAHS proteins form Tardigrade in osmotic stress tolerance in mammalian cells

This paper shows that tardigrade CAHS proteins form reversible aggregates upon hyperosmotic stimulation and confer stress resistance in HeLa cells.

The expression of the formin Fhod3 in mouse tongue striated muscle (jst.go.jp)

This paper shows the expression and subcellular localization of Fhod3, an actin polymerization factor, in striated muscle of the mouse tongue.

Cell-wide arrangement of Golgi/RE units depends on the microtubule organization (jst.go.jp)

This paper shows Golgi stacks gathered to form a "Golgi ring" surrounding the centric REs beneath the cilium in sea urchin and ascidian embryonic cells.

Molecular Alterations Associated with Pathophysiology in Liver-Specific ZO-1 and ZO-2 Knockout Mice (jst.go.jp)

This paper provides a comprehensive temporal analysis of molecular changes in ZO-1 and ZO-2 deficient livers, offering insights into the pathology associated with ZO deficiency.

Visualization of ER-to-Golgi trafficking of procollagen X (jst.go.jp)

This paper visualized the ER-to-Golgi transport of procollagen X, which is involved in cartilage remodeling, and analyzed the proteins required for its transport.

Deep learning-based segmentation of subcellular organelles in high-resolution phase-contrast images (jst.go.jp)

This paper shows the development of deep learning-based segmentation models for organelles in high-resolution phase-contrast images.

Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging (jst.go.jp)

This paper visualized the distribution of glycosyltransferases within the Golgi of mammalian cells and explored the determinants of their sub-Golgi localization.

Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells

This paper shows development of in vitro nuclear envelope reconstitution system using semi-intact cells.

A high-resolution phase-contrast microscopy system for label-free imaging in living cells

This paper shows a tuned-up phase-contrast microscopy system for visualizing dynamic organelle behaviors.

In vivo evidence for homeostatic regulation of ribosomal protein levels in Drosophila

Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease

This paper shows that the lysosomal membranes of cells derived from patients with Gaucher disease are susceptible to damage.

Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium

This paper shows that Coronin-1, which is an actin regulator, promotes cell rearrangement.

Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition

This paper shows that the activation of p38MAPK enhances CRP2 expression and induces phenotypic transition into myofibroblasts and that CRP2BP promotes transcription via the SRF/CArG-box as an adapter factor.

Visual quantification of prostaglandin E₂ discharge from a single cell

This paper shows the amount of prostaglandin E2 secreted from a single cell using a combination of imaging and simulation.

Rab32 and Rab38 maintain bone homeostasis by regulating intracellular traffic in osteoclasts

This paper shows that Rab32 and Rab38 are coordinately responsible for membrane trafficking that supports bone resorption in mouse osteoclasts.

Signal sequence-triage is activated by translocon obstruction sensed by an ER stress sensor IRE1α

This paper reports that translocon obstruction activates signal sequence-triage and the ER stress sensor IRE1α.

VAMP5 and distinct sets of cognate Q-SNAREs mediate exosome release

This paper shows that a novel MVB-localized SNARE VAMP5 regulates exosome release through the MVB-plasma membrane fusion.

FilGAP, a GAP for Rac1, down-regulates invadopodia formation in breast cancer cells

This paper shows that the RacGAP protein FilGAP is a negative regulator of invadopodia formation in breast cancer cells.

Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis

This paper shows sphingomyelin is an essential regulator of Ras excitability on the cell membrane that ensures efficient chemotaxis.

Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression

In this study, we have developed two fluorescent proteins of our own making to create Fucci5, which is free from license-related problems, in hopes of popularizing the Fucci technology in industry.

Proteomic Analysis of Fatty Liver Induced by Starvation of Medaka Fish Larvae

This paper shows the mechanism of starvation-induced development of fatty liver in medaka fish larvae analyzed by proteomic analysis.