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Antibody【Antigen:I-L】|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody【Antigen:I-L】

【I】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Iba12012-12-11 WBIF IHCIP
abcamx500
ab5076
4%PFA (in PBS)
goat
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
microglia
mouse brain
IL-1 receptor2011-08-29 12A6WBIF IHCIP
Pharmingenx200-500
rat
Mono
mouseCD121a, TypeI/p80
Hela細胞
Insulin2011-08-10 WBIF IHCIP
Sigmax100
I2018
3%PFA (in PBS)
mouse
m

マウス膵臓
Insulin2018-07-12 WBIF IHCIP
Cell Signaling1/10001/1001/400
L6B10
Mouse
IgG2a
Proinsulin is strongly hybridized with this antibody.
MIN6
IQGAP2011-09-27 WBIF IHCIP
BD100
I53820
mouse
m
TCA
HeLa
IRAK2011-08-29 7WBIF IHCIP
BD Transductionx500x500
mouse
Mono, IgG1

Hela細胞
IκB-α2011-08-29 C-21WBIF IHCIP
Santacruzx500x50-500
rabbit
Poly

Hela細胞
mouse I-A/I-E2011-08-10 WBIF IHCIP
BioLegend
107601
rat

mouse IgA2011-08-10 a chain specificWBIF IHCIP
Zymed
626700
goat

mouse IgD2011-08-10 WBIF IHCIP
BioLegend
405701
rat

mouse IgG2011-08-10 H+LWBIF IHCIP
Jackson
115007003
FabF goat

【J】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
JAM-A (JAM-1)2011-09-27 WBIF IHCIP
Zymed100
36-1700
Rabbit,
polyclonal
1%PFA, MeOH
DLD-1, EpH4
JIP12011-10-03 WBIF IHCIP
Santa Cruz×400 ×5000
rabbit
【Formalin in PBS+0.05%Triton】:○
Poly,M-300 sc-15353

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons

【K】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
human Ki672011-08-10 WBIF IHCIP
Novocastrax100
NCL-Ki67p
3%PFA (in PBS)
rabbit
p

マウス小腸
KAP2011-08-14 WBIF IHCIP
BD transductionx1000x100
610334
3%PFA (in PBS)
mouse
m

マウス神経細胞
Katanin p60 AL12018-07-26 A-10WBIF IHCIP
Santa Cruz Biotechnology1/100
sc-373814
MeOH fixation
Mouse
0.05% triton X-100

HeLa
KDEL2011-08-12 WBIF IHCIP
Stressgen
SPA827
mouse
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin
KDEL2011-08-10 WBIF IHCIP
Stressgenx100
SPA827
3%PFA (in PBS)
mouse
m

マウス膵、精巣
KDEL2011-09-17 10C3WBIF IHCIP
Stressgen
#SPA-827
mouse
Mono

Hela細胞
KDEL2011-09-28 10C3WBIF IHCIP
Stressgenx1000
#SPA-827
mouse
Mono

HeLa細胞
KDEL2011-11-26 SPA-827WBIF IHCIP
stressgenx50
ADI-SPA-827D
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
KDEL2012-08-03 WBIF IHCIP
Stressgenx100
SPA827
3%PFA in PBS
mouse
0.05% saponin

PC12
KDEL2013-01-19 10C3WBIF IHCIP
Stressgenx100
SPA827
3%PFA (in PBS)
mouse
0.05%saponin
IgG2a
弱いが、1/5くらいの細胞でそまる
Very weakly stained in -20% of all cells
Caco2細胞
KDEL receptor2011-08-14 WBIF IHCIP
Stressgenx100
VAA-PT048
3%PFA (in PBS)
mouse
m

マウス小腸
KDEL receptor2011-11-26 VAA-PT048WBIF IHCIP
stressgenx50
ADI-VAA-PT048D
4%PFA +0.1%Triton
mouse

Drosophila embryo, discs, nervous system
KDEL-R2011-08-29 VAA-PT048WBIF IHCIP
StressGenx200
mouse
Mono, IgG1

Hela細胞
Ki672012-12-11 WBIF IHCIP
Novocrastax200
NCL-Ki67-MM1
4%PFA (in PBS)
mouse
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
proliferation
mouse brain
Ki672012-12-11 WBIF IHCIP
Novocrastax200
NCL-Ki67P
4%PFA (in PBS)
rabbit
PFA-fixed cryosection/HistoVT one (nacalai) 70˚C 20min
IgG
proliferation
mouse brain
KIF13A2011-08-14 WBIF IHCIP
Zymed
52-5407

KIF13A2011-08-10 WBIF IHCIP
Zymedx100
525407
rabbit
p

【L】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
bovine LDLR2011-08-10 WBIF IHCIP
Progenx100
61087
3%PFA (in PBS), methanol
mouse
m

マウス小腸
FITC-Lotus2011-08-10 WBIF IHCIP
ホーネン  J-Oil Millsx100
300219
3%PFA (in PBS)
レクチン

マウス腎臓
human lamin B2011-08-10 C-20WBIF IHCIP
Santa Cruzx100
sc6216
3%PFA (in PBS)
goat
p

3T3, HeLa,マウス小腸、脾臓、脳など
human LAMP12011-08-10 H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA (in PBS)
mouse

Caco2細胞
human LAMP22011-08-10 H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA (in PBS)
mouse

Caco2細胞
human lysozyme2011-08-10 WBIF IHCIP
DakoCytomationx100
N1515
3%PFA (in PBS)
rabbit
p
Paneth cells are stained
マウス小腸
L12011-08-10 8D9WBIF IHCIP
DSHBx100
8D9
3%PFA (in PBS)
mouse

マウス脳
L12011-08-10 WBIF IHCIP
Chemiconx100
MAB5272
3%PFA (in PBS)
rat
m

マウス脳
L12011-08-12 8D9WBIF IHCIP
DSHB
8D9
mouse
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
L12011-08-12 WBIF IHCIP
Chemicon
MAB5272
rat
m
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
L12011-10-03 C20WBIF IHCIP
Santa cruzx1000
goat
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer液+0.05%Triton】:○
Poly

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
Lamin A+C2011-09-17
About Product
MonoclonalWBIF IHCIP
Abcamx1000
ab8984
mouse
IgG1

Hela細胞
Lamin A+C2011-09-28
About Product
MonoclonalWBIF IHCIP
Abcamx1000
ab8984
mouse
IgG1

HeLa細胞
Lamin A−Atto 647N2011-10-13 Atto647NWBIF IHCIP
SIGMAx100
L3544
rabbit
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
Poly

HeLa細胞
lamin C (Drosophila)2011-11-26 LC28.26WBIF IHCIP
Developmental study hybridoma bank C
4%PFA +0.1%Triton
mouse IgG
IgG

Drosophila embryo, discs, nervous system
lamin Dm0 (Drosophila)2011-11-26 ADL84.12WBIF IHCIP
Developmental study hybridoma bank Cx25
4%PFA +0.1%Triton
mouse IgG
IgG

Drosophila embryo, discs, nervous system
lamin Dm1,2(Drosophila)2011-11-26 ADL67.10WBIF IHCIP
Developmental study hybridoma bank Cx3000x25
4%PFA +0.1%Triton
mouse IgG
IgG

Drosophila embryo, discs, nervous system
LaminA/C2011-10-13 XB10WBIF IHCIP
covanceX2000
MMS-107P
mouse
MeOH
Mono, IgM

HeLa細胞
LaminB2011-10-13 101-B7WBIF IHCIP
Matri-TectX1000X500
M1100
mouse
MeOH
Mono, IgG1

HeLa細胞
LaminB12011-10-13 101- B7WBIF IHCIP
CALBIOCHEMX5000X500
NA12
mouse
MeOH
Mono, IgG1

HeLa細胞
Laminin 52011-09-29
About Product
PolyclonalWBIF IHCIP
Abcamx1,000x500
ab14509
rabbit
IF: -20℃ methanol or acetone:methanol=1:1
IgG

HeLa細胞
LAMP12011-09-27 H4A3WBIF IHCIP
Santa Cruz1:500~1:1000
sc-20011
mouse
m
Human細胞専用
human (HeLa, A549, MCF7)
LAMP12011-09-27 1D4BWBIF IHCIP
BD Biosciences1:100
553792
rat
m
mouse細胞専用
mouse (MEF, NIH3T3)
Lamp12011-10-06 1D4BWBIF IHCIP
eBiosciencex200
14-1071-81
Rat
4%PFA+0.1%Saponin :○
mono, IgG2a
マウスのみ
NIH3T3, マウス組織
Lamp12011-10-06 H4A3WBIF IHCIP
eBiosciencex400
14-1079-80
mouse
4%PFA+0.1%Saponin :○
IgG1
ヒトのみ
HeLa
LAMP12011-10-13 H4A3WBIF IHCIP
Santa CruzX200
sc-20011
mouse
Mono, IgG1

HT1080細胞
lamp12012-09-14 H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA in PBS
mouse
0.05% saponin
IgG1
もっと薄められる
Can be diluted more
Caco2
lamp12012-09-14 H4A3WBIF IHCIP
DSHBx100
H4A3
3%PFA in PBS
mouse
0.05% saponin
IgG1
H4A3, 1D4Bとも染まらず
Not stained by H4A3 or 1D4B Abs
MDCK
Lamp1 (CD107a)2011-08-29 H4B3WBIF IHCIP
BD Pharmingenx5000-10000
mouse
【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1k
MDCKII ダメ、saponin使うとかなり明るい。
Not suitable for MDCKII. Good staining by saponin permeabilization.
Hela細胞
lamp22012-09-14 H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA in PBS
mouse
0.05% saponin
IgG1
もっと薄められる
Can be diluted more
Caco2
lamp22012-09-14 H4B4WBIF IHCIP
DSHBx100
H4B4
3%PFA in PBS
mouse
0.05% saponin
IgG1
ABL93, H4B4, GL2A7全てダメ
Not stained by ABL93, H4A4 or GL2A7 Abs
MDCK
Lamp2 (CD107b)2011-08-29 H4B4WBIF IHCIP
BD Pharmingenx1000
mouse
【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
LAP22011-08-14 WBIF IHCIP
BD transductionx100
611000
3%PFA (in PBS)
mouse
m

培養細胞
LAR2011-08-14 WBIF IHCIP
BD transduction
L33420
mouse
m

LBPA2011-08-29 6C4WBIF IHCIP
Echelonx100
mouse
【PFA+Triton】:×,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1k

Hela細胞
LBPA(BMP)2016-12-14 6C4WBIF IHCIP
Echelon1/500
Z-PLBPA
4% PFA
Mouse
IgG

HeLa, PC12
LBR2011-10-13 E398LWBIF IHCIP
EPITOMICSx100
1398-1
rabbit
PFA (fix cells by adding 1/10 vol of 37% formalin directly to the culture medium)+Triton
Mono, IgG

HeLa細胞
LC32011-08-29 WBIF IHCIP
MBLx50
rabbit
【PFA_70%EtOH】:○【PFA条件】3%PFA/PBS 37℃10min
Poly

Hela細胞
LC32011-09-27 WBIF IHCIP
MBL1:10001:500~1:1000
PM036
rabbit
p

MEF ,NIH3T3, HeLa, A549, MCF7他
LC3B2011-08-10 WBIF IHCIP
sigma
L7543
rabbit
p

LDLR2011-08-29 C7WBIF IHCIP
ATCCダメ(?)x100
mouse
【PFA+Triton】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
mono
Hybridoma Supernatant
Hela細胞
Lefty-12011-09-22 WBIF IHCIP
R&D systems4 ug/ml
MAB9941
rat
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
Mab IgG2A

mouse E6.5 embryo
LGN2014-06-04 WBIF IHCIP
Bethyl Laboratoriesx1000
A303-032A
rabbit
IgG

HeLa
Kiyomitsu and Cheeseman, Cell 2013
luciferase2011-09-17 WBIF IHCIP
Promega
#G745A
goat
Poly

Hela細胞
luciferase2011-09-28 WBIF IHCIP
Promegax1000
#G745A
goat
Poly

HeLa細胞
mouse LAMP12011-08-10 1D4BWBIF IHCIP
DSHBx100
1D4B
3%PFA (in PBS)
rat
ImEMにも使用可能
Applicable for ImEM
マウス小腸
mouse LAMP22011-08-12 WBIF IHCIP
DSHB
ABL93
rat
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)染まらなかった
Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin
mouse LAMP22011-08-10 GL2A7WBIF IHCIP
DSHB
GL2A7
rat

mouse LAMP22011-08-10 ABL93WBIF IHCIP
DSHBx100
ABL93
3%PFA (in PBS)
rat
ImEMには使えない(GAで失活)が、PFAでは一番よい
Not suitable for ImEM because antigenicity is lost by GA. Stains LAMP2 best of all lamp2 Abs by PFA fix.
マウス小腸
mouse LDLR2011-08-10 NS0WBIF IHCIP
R&D Systemsx1000x100
AF2255
3%PFA (in PBS)
goat

マウス小腸、腎臓
mouse LDLR2011-08-10 M-20WBIF IHCIP
Santa Cruzx100
sc11826
3%PFA (in PBS), methanol
goat
p

マウス小腸
mouse Ly-6G/Ly-6C2011-08-10 Gr-1WBIF IHCIP
BioLegend
108401
rat

ovine LHb2011-08-10 WBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACOV27b01RBP85
3%PFA (in PBS)
rabbit

マウス下垂体