日本細胞生物学会Japan Society for Cell Biology

Immunofluorescence of tissue sections(5〜10μm)

AuthorAkihiro Harada
AffiliationDepartment of Cell Biology, Graduate School of Medicine, Osaka University
Contactinquiry about this protocol
Home Pagehttp://www.med.osaka-u.ac.jp/pub/acb/eng.html
Keyword
Published2015-08-26Last Update2015-08-26
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Summary

Methods

Detail  *Click photo to see a large size.

    • Washi with PBS 5min×3(to remove OCT compound)
    • 5%Normal donkey serum in PBS 5-10min
    • diluted primary antibody 37℃1hr
    • Wash with PBS 5min×3
    • 5%Normal donkey serum in PBS 5-10min
    • diluted secondary antibody RT1hr
    • Wash with PBS, 5min×3
    • embedding
      A put a drop of mounting medium (or PBS) onto the section on slide and cover the section with a coverslip.
        B seal the coverslip with nail polish 5%Normal donkey serum in PBS Normal donkey serum(CHEMICON S30-100ML) Aliquot and store at -20℃. Dilute with PBS to 5% on usage.

Little trick

Reference